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This volume provides methods and approaches to study genetic and environmental regulatory controls on odontogenesis. Chapters guide readers through protocols for isolation and characterization of both epithelial and mesenchymal dental cells, methods on isolation, phenotypic characterization, expansion, differentiation, immunofluorescence, in situ hybridization, immunohistochemistry, imaging protocols, rodent dental fluorosis model, 3D assessment of crown size, dental diseases models, next generation sequencing, genetic and epigenetic studies, genome-wide association studies as well as clinical protocols for measurement of early childhood caries and saliva, and supragingival fluids and biofilm collection and subsequent analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Odontogenesis: Methods and Protocols aims to guide researchers towards elucidating the secrets and mysteries of a fascinating and unique organ, the tooth. Author Information Dr. Petros Papagerakis graduated from the School of Dentistry-Aristotle University, Thessaloniki, Greece. He completed postgraduate training at the University of Paris (France) in Pediatric and Preventive Dentistry, Hospital Pediatric Dentistry, Biomedical Sciences (BSc), Oral Biology (MSc), and Cellular and Developmental Biology (PhD). He also completed a NIH-funded post-doctoral training in Craniofacial Molecular Genetics at the University of Texas, USA. Petros served as faculty of Dentistry (primary) and Medicine (affiliated) at the 7th University of Paris, the University of Texas and the University of Michigan before his recent relocation at the University of Saskatchewan.
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